Hydrogen inhalation ameliorates lung inflammation in mice with asthma
Li H et al. · Eur J Med Res. 2025;30:1024.
What kind of evidence is this?
Preclinical
Randomized mouse ovalbumin-asthma experiment
Respiratory health
Other forms
Not reported in this record.
Not reported in this record.
Methods at a glance
Female C57BL/6 mice sensitized and challenged with ovalbumin
32 mice; the article describes groups of eight
One hour daily for 7 consecutive days
Whole-body exposure to 15% H₂ in air for 1 hour daily over 7 consecutive days
H₂ diluted in air — not Brown’s gas.
15% H₂ in air at a reported total chamber flow of 4 L/min.
600 mL/min H₂ — calculated from 15% H₂ in the reported 4 L/min total mixture.
No separate O₂ stream or O₂ flow is reported; the balance is described as air.
Ovalbumin-asthma mice exposed to ambient atmosphere, plus non-asthmatic controls
Outcomes and reported result
Airway responsiveness, type-2 cytokines, IgE, regulatory cytokines, lung histology, MDA and SOD
The article reports lower airway responsiveness, inflammation, IgE and MDA and higher SOD and regulatory-cytokine expression with H₂.
Allocation, concentration, total flow, timing, outcomes, funding and conflicts checked in the open-access full text.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Small preclinical asthma model. The 600 mL/min figure is a calculated H₂ component of chamber flow, not a separately measured animal inspired-flow value.
Ovalbumin-induced airway inflammation in mice, not clinical asthma.
Not reported in this record.
Not reported in this record.
Not reported in this record.
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 41146240 · DOI: 10.1186/s40001-025-03296-7
Open-access full text is available through PMC and the publisher.
Open-access publisher/PMC full text and PubMed record; no retraction or expression of concern was shown in the checked records on 7 August 2026.
2026-08-10