The effect of hydrogen-rich saline solution on erythrocyte deformability in lower limb ischemia reperfusion injury in rats
Özer A, Arslan M, Çomu FM, Küçük A, Mardin B, Koçak B, Oktar GL. · Gazi Med J. 2021;32:270–272.
Study at a glance
Preclinical
Male Wistar rats in a lower-limb ischemia/reperfusion model.
Intraperitoneal hydrogen-rich saline 30 minutes before ischemia. · The article reports 20 mg/kg, an unusual mass-based expression for the saline intervention.
120 minutes ischemia plus 120 minutes reperfusion.
The article reports worse deformability after ischemia/reperfusion and a lower impairment measure with hydrogen-rich saline.
The internal inconsistency in total animals and groups materially reduces confidence.
What kind of evidence is this?
Preclinical
Randomized controlled rat study
Critical care and ischemia-reperfusion
Other forms
Positive preclinical signal with serious reporting inconsistency.
Ex-vivo erythrocyte rheology after animal ischemia/reperfusion.
Methods
Male Wistar rats in a lower-limb ischemia/reperfusion model.
The abstract states 18 rats in three groups, while the methods state 24 rats and four groups but describe only three; the discrepancy is unresolved.
120 minutes ischemia plus 120 minutes reperfusion.
Intraperitoneal hydrogen-rich saline 30 minutes before ischemia.
H₂ dissolved in saline.
The article reports 20 mg/kg, an unusual mass-based expression for the saline intervention.
Control and ischemia/reperfusion groups.
Outcomes and reported result
Erythrocyte deformability.
The article reports worse deformability after ischemia/reperfusion and a lower impairment measure with hydrogen-rich saline.
The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
The internal inconsistency in total animals and groups materially reduces confidence.
The reported rat ischemia/reperfusion model only.
SYRCLE animal-study tool — preliminary
High risk
Conflicting sample/group reporting, unclear concealment and unclear blinding create high risk of bias.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
DOI: 10.12996/gmj.2021.62 · DOI: 10.12996/gmj.2021.62
A free publisher or repository full article was checked.
Publisher or repository full article and bibliographic record; checked 10 August 2026.
10 August 2026
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