Radio-protective effect of hydrogen rich water combined with amifostine in mice
Qin X, Yin J, Li J, Qin A, Wen J, Niu QL. · International Journal of Radiation Research. 2016;14(2):113–118.
What kind of evidence is this?
Preclinical
Controlled mouse irradiation study
Cancer research
H₂-rich water
Not reported in this record.
Not reported in this record.
Methods at a glance
Male ICR mice exposed to 9.0 Gy whole-body irradiation.
48 mice: control n=8; irradiation, amifostine plus irradiation, hydrogen-rich water plus irradiation, and combined treatment groups n=10 each.
Single pretreatment 30 minutes before irradiation; survival followed for 30 days.
Hydrogen-rich water 20 mL/kg by intragastric administration 30 minutes before irradiation; combination group also received amifostine 400 mg/kg intraperitoneally.
H₂ dissolved in water — H₂ only, not Brown's gas.
Water was prepared under 0.4 MPa hydrogen overnight and contained more than 0.5 mmol/L H₂.
Not applicable — intragastric water, not gas inhalation.
No O₂ was co-delivered.
Irradiation alone, amifostine alone plus irradiation, hydrogen-rich water alone plus irradiation, and non-irradiated control.
Outcomes and reported result
30-day survival and radiation-related mortality.
Thirty-day survival was 50% after irradiation, 70% with amifostine, 80% with hydrogen-rich water and 100% with the combination. Only the combined group differed significantly from irradiation alone; the single-agent comparisons were not significant.
The free full article, methods, survival figure and null single-agent comparisons were checked.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Preclinical radiation model. The apparent survival difference for hydrogen-rich water alone was not statistically significant; the study does not establish radioprotection in people.
Whole-body irradiation in male ICR mice only.
Not reported in this record.
Not reported in this record.
Not reported in this record.
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
OpenAlex ID: W2514157330 · DOI: 10.18869/acadpub.ijrr.14.2.113
Free journal PDF.
Journal full article; full-text extraction checked 8 August 2026.
2026-08-10