Hydrogen-Rich Water Prevents Dehydration-Induced Cellular Oxidative Stress and Cell Death in Human Skin Keratinocytes
Xiao L, Miwa N. · Hydrogen. 2022;3(1):20–31.
Study at a glance
Preclinical
HaCaT human keratinocyte cell cultures subjected to controlled air drying.
Cells were pretreated for 2 hours with hydrogen-water-prepared medium, then air-dried for 5, 10 or 20 minutes at 22–24°C and 35–40% humidity. · Hydrogen-water DMEM contained approximately 460 µg/L dissolved H₂. Source water measured 880±38 ppb at 24°C; boiled/cooled source water measured 420±25 ppb.
2-hour pretreatment followed by 5–20 minutes of air drying.
After 20 minutes of drying, viability was 27.6% in control medium and 61.1% after hydrogen-rich-medium pretreatment (p<0.001). Five-minute drying ROS signals were also lower with hydrogen-rich medium.
In-vitro evidence only. HaCaT cell results do not establish a skin treatment effect in people. The authors reported no conflict of interest.
What kind of evidence is this?
Preclinical
Controlled in-vitro study
Skin, wound and aging research
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
HaCaT human keratinocyte cell cultures subjected to controlled air drying.
Cell-culture experiments; the article reports independent assays rather than one consolidated participant or animal sample.
2-hour pretreatment followed by 5–20 minutes of air drying.
Cells were pretreated for 2 hours with hydrogen-water-prepared medium, then air-dried for 5, 10 or 20 minutes at 22–24°C and 35–40% humidity.
H₂ dissolved in cell-culture medium — H₂ only, not Brown's gas.
Hydrogen-water DMEM contained approximately 460 µg/L dissolved H₂. Source water measured 880±38 ppb at 24°C; boiled/cooled source water measured 420±25 ppb.
Distilled/deionized-water-prepared medium.
Outcomes and reported result
Cell viability and nuclear/cytoplasmic reactive-oxygen-species fluorescence after dehydration stress.
After 20 minutes of drying, viability was 27.6% in control medium and 61.1% after hydrogen-rich-medium pretreatment (p<0.001). Five-minute drying ROS signals were also lower with hydrogen-rich medium.
The free publisher full article, methods, figures and conflict statement were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
In-vitro evidence only. HaCaT cell results do not establish a skin treatment effect in people. The authors reported no conflict of interest.
Cultured human keratinocytes under the reported dehydration protocol.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
OpenAlex ID: W4225080080 · DOI: 10.3390/hydrogen3010005
Open-access full article on MDPI.
MDPI publisher full article; full-text extraction checked 8 August 2026.
10 August 2026
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