Hydrogenology
Hydrogenology editorial study record

The Anti-inflammatory Effects of Hydrogen-Rich Water Acts in a Volume-Dependent Manner on Rats' Lungs Exposed to Cigarette Smoke

Rahmatullah AS, Santoso A, Cangara MH, Yustisia I, Aminuddin, Adnan E. · JUMANTIK. 2023;8(2):197–206.

PreclinicalH₂-rich waterPublished 2023
Study record, not medical adviceThis page reports one source and Hydrogenology’s source-text extraction. It does not establish that molecular hydrogen is effective, safe or appropriate for any person. Human, animal and laboratory evidence must not be treated as equivalent.
Source and classification

What kind of evidence is this?

Evidence stream

Preclinical

Reported design

Randomized controlled rat study

Research topic

Respiratory health

Administration classification

H₂-rich water

Study result signal

Not reported in this record.

Outcome type

Not reported in this record.

Reported in the source

Methods at a glance

Population or model

Male Wistar rats exposed to free air or cigarette smoke.

Sample

20 rats randomized to four groups; n=5 per group.

Duration

28 days.

Intervention

Oral hydrogen-rich water 5 mL once daily or 5 mL twice daily, given 30 minutes before exposure to five cigarettes/day for 28 days.

Hydrogen form

H₂ dissolved in drinking water — H₂ only, not Brown's gas.

H₂ specification

1.0–1.5 ppm dissolved H₂, measured with a hydrogen meter after preparation.

H₂ flow

Not applicable — oral water, not gas inhalation.

O₂ delivered with H₂

No O₂ was co-delivered.

Comparator

Water plus free air, water plus cigarette smoke, and two hydrogen-rich-water dosing groups plus cigarette smoke.

Reported, not endorsed

Outcomes and reported result

Outcomes measured

Lung NF-κB p65 by ELISA and semi-quantitative histopathological inflammation score.

Reported result

The twice-daily 5 mL group had lower NF-κB p65 than cigarette-smoke control (p<0.01). Both hydrogen-water groups had lower histopathological inflammation scores than smoke control (p<0.05).

Results-extraction completeness

The complete free journal full article, methods, tables and histopathology results were checked.

A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.

Interpretation limits

Cautions and applicability

Design and reporting cautions

Five animals per group, preclinical smoke-exposure model and subjective histopathology scoring. Results do not establish treatment of smoking-related disease in people.

Applies directly to

Cigarette-smoke-exposed rats only.

Preliminary appraisal framework

Not reported in this record.

Preliminary risk-of-bias status

Not reported in this record.

Appraisal rationale

Not reported in this record.

No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.

Provenance

Sources and record status

Identifiers

OpenAlex ID: W4385487070 · DOI: 10.30829/jumantik.v8i2.14790

Publisher access

Open-access journal article.

Extraction basis

JUMANTIK full article; full-text extraction checked 8 August 2026.

Record revision

2026-08-10

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