The Anti-inflammatory Effects of Hydrogen-Rich Water Acts in a Volume-Dependent Manner on Rats' Lungs Exposed to Cigarette Smoke
Rahmatullah AS, Santoso A, Cangara MH, Yustisia I, Aminuddin, Adnan E. · JUMANTIK. 2023;8(2):197–206.
Study at a glance
Preclinical
Male Wistar rats exposed to free air or cigarette smoke.
Oral hydrogen-rich water 5 mL once daily or 5 mL twice daily, given 30 minutes before exposure to five cigarettes/day for 28 days. · 1.0–1.5 ppm dissolved H₂, measured with a hydrogen meter after preparation.
28 days.
The twice-daily 5 mL group had lower NF-κB p65 than cigarette-smoke control (p<0.01). Both hydrogen-water groups had lower histopathological inflammation scores than smoke control (p<0.05).
Five animals per group, preclinical smoke-exposure model and subjective histopathology scoring. Results do not establish treatment of smoking-related disease in people.
What kind of evidence is this?
Preclinical
Randomized controlled rat study
Respiratory health
H₂-rich water
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Male Wistar rats exposed to free air or cigarette smoke.
20 rats randomized to four groups; n=5 per group.
28 days.
Oral hydrogen-rich water 5 mL once daily or 5 mL twice daily, given 30 minutes before exposure to five cigarettes/day for 28 days.
H₂ dissolved in drinking water — H₂ only, not Brown's gas.
1.0–1.5 ppm dissolved H₂, measured with a hydrogen meter after preparation.
Water plus free air, water plus cigarette smoke, and two hydrogen-rich-water dosing groups plus cigarette smoke.
Outcomes and reported result
Lung NF-κB p65 by ELISA and semi-quantitative histopathological inflammation score.
The twice-daily 5 mL group had lower NF-κB p65 than cigarette-smoke control (p<0.01). Both hydrogen-water groups had lower histopathological inflammation scores than smoke control (p<0.05).
The complete free journal full article, methods, tables and histopathology results were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Five animals per group, preclinical smoke-exposure model and subjective histopathology scoring. Results do not establish treatment of smoking-related disease in people.
Cigarette-smoke-exposed rats only.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
OpenAlex ID: W4385487070 · DOI: 10.30829/jumantik.v8i2.14790
Open-access journal article.
JUMANTIK full article; full-text extraction checked 8 August 2026.
10 August 2026
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