Effect of hydrogen inhalation on cardiovascular and interstitial components of pulmonary hypertension in rats
Kuropatkina TA, Gufranov K, Sychev FY, et al. · Pulmonologiya. 2024;34(1):19–30.
What kind of evidence is this?
Preclinical
Controlled monocrotaline pulmonary-hypertension rat study
Respiratory health
Inhaled H₂
Not reported in this record.
Not reported in this record.
Methods at a glance
Male Wistar rats with monocrotaline-induced pulmonary hypertension.
Outcome table reports control n=7, monocrotaline control n=6 and monocrotaline plus H₂ n=6.
Continuous exposure for 21 days.
Continuous exposure to nominal 4% H₂ in air for 21 days after monocrotaline administration.
H₂ mixed with room air — H₂ alone from the generator, not Brown's gas.
Outlet concentration was 4.1±0.3% H₂. The methods list 150 mL/min H₂ and 4 mL/min air; those printed flows cannot mathematically produce 4% H₂, so Hydrogenology does not reconcile them.
150 mL/min H₂ is printed in the methods, but it is internally inconsistent with the printed air flow and measured 4.1% concentration.
No supplemental O₂ was co-delivered; room air was the diluent. The full article does not report an absolute O₂ flow.
Monocrotaline plus room air and solvent-only control groups.
Outcomes and reported result
Right-ventricular pressure and hypertrophy, lung histology, mast cells, fibrosis and TGF-β immunostaining.
Hydrogen did not change the principal pulmonary-hypertension cardiovascular measures: right-ventricular systolic pressure was 57±9 versus 58±7 mmHg. Mast-cell counts and several inflammatory/fibrotic lung findings were lower with hydrogen.
The complete free publisher full article, methods, tables, positive and null results were checked.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Preclinical model. No benefit was found for the principal cardiovascular manifestations of pulmonary hypertension. The article contains an unresolved flow inconsistency: 150 mL/min H₂ plus 4 mL/min air cannot yield the stated 4.1% H₂.
Monocrotaline pulmonary-hypertension model in rats only.
Not reported in this record.
Not reported in this record.
Not reported in this record.
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
OpenAlex ID: W4392132150 · DOI: 10.18093/0869-0189-2024-34-1-19-30
Open-access publisher article under CC BY-NC 4.0.
Pulmonologiya full article; full-text extraction checked 8 August 2026.
2026-08-10