Hydrogen-rich saline attenuates vascular smooth muscle cell proliferation and neointimal hyperplasia by inhibiting reactive oxygen species production and inactivating the Ras–ERK1/2–MEK1/2 and Akt pathways
Chen Y, Jiang J, Miao H, Chen X, Sun X, Li Y. · Int J Mol Med. 2013;31(3):597–606.
Study at a glance
Preclinical
Rats after carotid balloon injury and cultured rat vascular smooth-muscle cells.
Daily hydrogen-rich saline in rats and hydrogen-rich culture medium in cell experiments. · Full preparation and exposure details are reported in the checked article.
Article-specific animal and cell time points.
The article reports less neointimal hyperplasia and lower stimulated cell proliferation, migration, ROS and pathway activation with the H₂ preparations.
Preclinical evidence only; cell and animal findings do not establish patient benefit.
What kind of evidence is this?
Preclinical
Controlled rat and cell study
Cardiovascular and circulatory health
Other forms
Positive preclinical signal.
Vascular histology and mechanistic cell outcomes.
Methods
Rats after carotid balloon injury and cultured rat vascular smooth-muscle cells.
Animal group and cell-assay counts were checked in the full article.
Article-specific animal and cell time points.
Daily hydrogen-rich saline in rats and hydrogen-rich culture medium in cell experiments.
H₂ dissolved in saline or culture medium.
Full preparation and exposure details are reported in the checked article.
Vehicle-treated injured rats and conventional culture medium.
Outcomes and reported result
Neointimal hyperplasia, cell proliferation and migration, ROS and Ras/ERK/MEK/Akt signaling.
The article reports less neointimal hyperplasia and lower stimulated cell proliferation, migration, ROS and pathway activation with the H₂ preparations.
The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical evidence only; cell and animal findings do not establish patient benefit.
The reported balloon-injury and cell models only.
SYRCLE animal-study tool — preliminary
Some concerns
Comparator experiments were reported, but allocation concealment and blinded outcome assessment were unclear.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 23340693 · DOI: 10.3892/ijmm.2013.1256
A free publisher or repository full article was checked.
Publisher or repository full article and bibliographic record; checked 10 August 2026.
10 August 2026
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