Hydrogen-rich saline attenuates vascular smooth muscle cell proliferation and neointimal hyperplasia by inhibiting reactive oxygen species production and inactivating the Ras–ERK1/2–MEK1/2 and Akt pathways
Chen Y, Jiang J, Miao H, Chen X, Sun X, Li Y. · Int J Mol Med. 2013;31(3):597–606.
What kind of evidence is this?
Preclinical
Controlled rat and cell study
Cardiovascular and circulatory health
Other forms
Positive preclinical signal.
Vascular histology and mechanistic cell outcomes.
Methods at a glance
Rats after carotid balloon injury and cultured rat vascular smooth-muscle cells.
Animal group and cell-assay counts were checked in the full article.
Article-specific animal and cell time points.
Daily hydrogen-rich saline in rats and hydrogen-rich culture medium in cell experiments.
H₂ dissolved in saline or culture medium.
Full preparation and exposure details are reported in the checked article.
Not applicable.
No oxygen was co-delivered.
Vehicle-treated injured rats and conventional culture medium.
Outcomes and reported result
Neointimal hyperplasia, cell proliferation and migration, ROS and Ras/ERK/MEK/Akt signaling.
The article reports less neointimal hyperplasia and lower stimulated cell proliferation, migration, ROS and pathway activation with the H₂ preparations.
The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Preclinical evidence only; cell and animal findings do not establish patient benefit.
The reported balloon-injury and cell models only.
SYRCLE animal-study tool — preliminary
Some concerns
Comparator experiments were reported, but allocation concealment and blinded outcome assessment were unclear.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 23340693 · DOI: 10.3892/ijmm.2013.1256
A free publisher or repository full article was checked.
Publisher or repository full article and bibliographic record; checked 10 August 2026.
2026-08-10