Hydrogen-rich saline attenuated neuropathic pain by reducing oxidative stress
Chen Q, Chen P, Zhou S, Yan X, Zhang J, Sun X, Yuan H, Yu W. · Can J Neurol Sci. 2013;40(6):857–863.
Study at a glance
Preclinical
Sprague-Dawley rats with chronic constriction injury.
Intrathecal hydrogen-rich saline, 100 μL/kg daily on days 1–7. · At least 0.6 mmol/L dissolved H₂.
Seven treatment days with behavioral follow-up through day 14.
Withdrawal thresholds and several oxidative and signaling measures favored hydrogen-rich saline; P2X4R did not differ.
Animal evidence only; multiple outcomes and limited blinding information.
What kind of evidence is this?
Preclinical
Randomized controlled rat study
Musculoskeletal and pain research
Other forms
Mixed positive preclinical signal.
Behavioral and spinal-cord biomarker outcomes in a neuropathic-pain model.
Methods
Sprague-Dawley rats with chronic constriction injury.
30 rats; three groups of 10.
Seven treatment days with behavioral follow-up through day 14.
Intrathecal hydrogen-rich saline, 100 μL/kg daily on days 1–7.
H₂ dissolved in saline.
At least 0.6 mmol/L dissolved H₂.
Sham and constriction-injury groups receiving physiological saline.
Outcomes and reported result
Mechanical withdrawal threshold, spinal oxidative markers and p38 MAPK, BDNF and P2X4R expression.
Withdrawal thresholds and several oxidative and signaling measures favored hydrogen-rich saline; P2X4R did not differ.
The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Animal evidence only; multiple outcomes and limited blinding information.
The reported rat constriction-injury model only.
SYRCLE animal-study tool — preliminary
Some concerns
Random allocation was stated, but concealment and blinded outcome assessment were not established.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 24257230 · DOI: 10.1017/s0317167100016024
A free publisher or repository full article was checked.
Publisher or repository full article and bibliographic record; checked 10 August 2026.
10 August 2026
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