Protective role of hydrogen-rich water on aspirin-induced gastric mucosal damage in rats
Zhang JY, Wu QF, Wan Y, et al. · World Journal of Gastroenterology. 2014;20(6):1614–1622.
Study at a glance
Preclinical
Male Sprague-Dawley rats in an aspirin-induced gastric mucosal injury model.
Hydrogen-rich water was provided for 7 days, refreshed every 3 hours at approximately 80 mL/day per rat; aspirin groups received 400 mg/kg on day 8. · 0.63–0.82 mmol/L dissolved H₂, confirmed by gas chromatography.
7-day pretreatment; tissues collected 8 hours after aspirin on day 8.
Hydrogen-rich-water pretreatment reduced the aspirin-associated damage score from 4.04±0.492 to 2.10±0.437 (p<0.05), with lower MDA, MPO, inflammatory cytokines and COX-2 staining and higher SOD than aspirin alone.
Preclinical acute injury model; it does not establish prevention or treatment efficacy in people. Multiple laboratory outcomes were assessed.
What kind of evidence is this?
Preclinical
Randomized controlled rat study
Gastrointestinal health
H₂-rich water
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Male Sprague-Dawley rats in an aspirin-induced gastric mucosal injury model.
40 rats randomized to four groups; n=10 per group.
7-day pretreatment; tissues collected 8 hours after aspirin on day 8.
Hydrogen-rich water was provided for 7 days, refreshed every 3 hours at approximately 80 mL/day per rat; aspirin groups received 400 mg/kg on day 8.
H₂ dissolved in drinking water — H₂ only, not Brown's gas.
0.63–0.82 mmol/L dissolved H₂, confirmed by gas chromatography.
Normal control, hydrogen-water control, aspirin, and hydrogen-rich-water plus aspirin groups.
Outcomes and reported result
Gastric mucosal damage score, histology, MDA, MPO, SOD, cytokines and COX-2 staining.
Hydrogen-rich-water pretreatment reduced the aspirin-associated damage score from 4.04±0.492 to 2.10±0.437 (p<0.05), with lower MDA, MPO, inflammatory cytokines and COX-2 staining and higher SOD than aspirin alone.
The complete free PMC article, methods, tables, figures and results were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical acute injury model; it does not establish prevention or treatment efficacy in people. Multiple laboratory outcomes were assessed.
Aspirin-induced gastric mucosal injury in rats only.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 24587639 · DOI: 10.3748/wjg.v20.i6.1614
Free full article on PubMed Central.
PubMed Central full article and PubMed record; full-text extraction checked 8 August 2026.
10 August 2026
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