Hydrogenology
Hydrogenology editorial study record

Protective role of hydrogen-rich water on aspirin-induced gastric mucosal damage in rats

Zhang JY, Wu QF, Wan Y, et al. · World Journal of Gastroenterology. 2014;20(6):1614–1622.

PreclinicalH₂-rich waterPublished 2014
Study record, not medical adviceThis page reports one source and Hydrogenology’s source-text extraction. It does not establish that molecular hydrogen is effective, safe or appropriate for any person. Human, animal and laboratory evidence must not be treated as equivalent.
Source and classification

What kind of evidence is this?

Evidence stream

Preclinical

Reported design

Randomized controlled rat study

Research topic

Gastrointestinal health

Administration classification

H₂-rich water

Study result signal

Not reported in this record.

Outcome type

Not reported in this record.

Reported in the source

Methods at a glance

Population or model

Male Sprague-Dawley rats in an aspirin-induced gastric mucosal injury model.

Sample

40 rats randomized to four groups; n=10 per group.

Duration

7-day pretreatment; tissues collected 8 hours after aspirin on day 8.

Intervention

Hydrogen-rich water was provided for 7 days, refreshed every 3 hours at approximately 80 mL/day per rat; aspirin groups received 400 mg/kg on day 8.

Hydrogen form

H₂ dissolved in drinking water — H₂ only, not Brown's gas.

H₂ specification

0.63–0.82 mmol/L dissolved H₂, confirmed by gas chromatography.

H₂ flow

Not applicable — drinking water, not gas inhalation.

O₂ delivered with H₂

No O₂ was co-delivered.

Comparator

Normal control, hydrogen-water control, aspirin, and hydrogen-rich-water plus aspirin groups.

Reported, not endorsed

Outcomes and reported result

Outcomes measured

Gastric mucosal damage score, histology, MDA, MPO, SOD, cytokines and COX-2 staining.

Reported result

Hydrogen-rich-water pretreatment reduced the aspirin-associated damage score from 4.04±0.492 to 2.10±0.437 (p<0.05), with lower MDA, MPO, inflammatory cytokines and COX-2 staining and higher SOD than aspirin alone.

Results-extraction completeness

The complete free PMC article, methods, tables, figures and results were checked.

A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.

Interpretation limits

Cautions and applicability

Design and reporting cautions

Preclinical acute injury model; it does not establish prevention or treatment efficacy in people. Multiple laboratory outcomes were assessed.

Applies directly to

Aspirin-induced gastric mucosal injury in rats only.

Preliminary appraisal framework

Not reported in this record.

Preliminary risk-of-bias status

Not reported in this record.

Appraisal rationale

Not reported in this record.

No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.

Provenance

Sources and record status

Identifiers

PMID: 24587639 · DOI: 10.3748/wjg.v20.i6.1614

Publisher access

Free full article on PubMed Central.

Extraction basis

PubMed Central full article and PubMed record; full-text extraction checked 8 August 2026.

Record revision

2026-08-10

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