Hydrogen treatment protects against cell death and senescence induced by oxidative damage
Han AL, Park SH, Park MS. · J Microbiol Biotechnol. 2017;27(2):365–371.
Study at a glance
Preclinical
Murine embryonic fibroblasts exposed to oxidative or replicative stress.
Hydrogen nanoparticle water added to cell culture. · Reported concentrations approximately 400–1,800 ppb across experiments.
Four hours to eight days depending on assay.
Several viability, proliferation, ROS and senescence measures favored hydrogen-treated cultures.
Cell-only evidence and a nanoparticle preparation with limited independent characterization.
What kind of evidence is this?
Preclinical
Controlled cell-culture study
Skin, wound and aging research
Other forms
Positive cell-culture signal.
Cell viability, senescence and oxidative-stress outcomes.
Methods
Murine embryonic fibroblasts exposed to oxidative or replicative stress.
Three to four independent measurements for reported assays.
Four hours to eight days depending on assay.
Hydrogen nanoparticle water added to cell culture.
H₂ nanoparticles dispersed in water and culture medium.
Reported concentrations approximately 400–1,800 ppb across experiments.
Conventional culture with or without hydroxyurea or hydrogen peroxide.
Outcomes and reported result
Cell proliferation, viability, LDH, senescence, ROS and nuclear morphology.
Several viability, proliferation, ROS and senescence measures favored hydrogen-treated cultures.
The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Cell-only evidence and a nanoparticle preparation with limited independent characterization.
The tested fibroblast cultures only.
In-vitro study appraisal — preliminary
Some concerns
Small replicate counts, multiple assays and generally unreported blinding.
Independent replicate reporting checked · Exposure and comparator checked · Assay blinding generally unreported · Multiplicity and selective reporting remain possible · External validity is limited to the tested cells
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 27780950 · DOI: 10.4014/jmb.1608.08011
A free publisher or repository full article was checked.
Publisher or repository full article and bibliographic record; checked 10 August 2026.
10 August 2026
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