Hydrogen treatment protects against cell death and senescence induced by oxidative damage
Han AL, Park SH, Park MS. · J Microbiol Biotechnol. 2017;27(2):365–371.
What kind of evidence is this?
Preclinical
Controlled cell-culture study
Skin, wound and aging research
Other forms
Positive cell-culture signal.
Cell viability, senescence and oxidative-stress outcomes.
Methods at a glance
Murine embryonic fibroblasts exposed to oxidative or replicative stress.
Three to four independent measurements for reported assays.
Four hours to eight days depending on assay.
Hydrogen nanoparticle water added to cell culture.
H₂ nanoparticles dispersed in water and culture medium.
Reported concentrations approximately 400–1,800 ppb across experiments.
Not applicable.
No oxygen was co-delivered.
Conventional culture with or without hydroxyurea or hydrogen peroxide.
Outcomes and reported result
Cell proliferation, viability, LDH, senescence, ROS and nuclear morphology.
Several viability, proliferation, ROS and senescence measures favored hydrogen-treated cultures.
The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Cell-only evidence and a nanoparticle preparation with limited independent characterization.
The tested fibroblast cultures only.
In-vitro study appraisal — preliminary
Some concerns
Small replicate counts, multiple assays and generally unreported blinding.
Independent replicate reporting checked · Exposure and comparator checked · Assay blinding generally unreported · Multiplicity and selective reporting remain possible · External validity is limited to the tested cells
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 27780950 · DOI: 10.4014/jmb.1608.08011
A free publisher or repository full article was checked.
Publisher or repository full article and bibliographic record; checked 10 August 2026.
2026-08-10