Preventive effects of drinking hydrogen-rich water on gingival oxidative stress and alveolar bone resorption in rats fed a high-fat diet
Yoneda T, Tomofuji T, Kunitomo M, Ekuni D, Irie K, Azuma T, Machida T, Miyai H, Fujimori K, Morita M. · Nutrients. 2017;9(1):64.
Study at a glance
Preclinical
18 male Fischer-344 rats assigned at six weeks of age.
Fresh electrolyzed H₂-rich drinking water supplied in a closed glass vessel and replaced every 12 hours until age 20 weeks. · Measured H₂ was 301.7±65.1 μg/L one minute after electrolysis and 186.3±55 μg/L after 24 hours; water intake volume was not reported.
From age six to 20 weeks, with water replaced twice daily.
Body-weight gain, gingival/serum oxidative markers and selected alveolar-bone measures favored H₂ water versus high-fat diet alone. Serum cholesterol/triglyceride comparisons between the two high-fat groups and trabecular number/thickness were not significant.
Only six male rats per group, high-fat model without induced periodontitis, unquantified water intake, no non-H₂ electrolyzed-water control and possible mediation by lower weight gain. Japanese public grants funded the work; authors declared no conflict and identified the commercial electrolyzer model.
What kind of evidence is this?
Preclinical
Randomized three-group rat high-fat-diet and drinking-water experiment
Oral and dental health
H₂-rich water
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
18 male Fischer-344 rats assigned at six weeks of age.
18 rats, n=6 per group: regular diet plus distilled water, high-fat diet plus distilled water, and high-fat diet plus H₂-rich water.
From age six to 20 weeks, with water replaced twice daily.
Fresh electrolyzed H₂-rich drinking water supplied in a closed glass vessel and replaced every 12 hours until age 20 weeks.
H₂ dissolved in drinking water — H₂ only, not Brown's gas and not inhalation.
Measured H₂ was 301.7±65.1 μg/L one minute after electrolysis and 186.3±55 μg/L after 24 hours; water intake volume was not reported.
High-fat diet plus distilled water, and regular diet plus distilled water; no high-fat group received non-H₂ electrolyzed water.
Outcomes and reported result
Body weight, gingival and serum 8-OHdG, serum lipids, alveolar-bone mineral/microarchitecture and tooth–bone distance.
Body-weight gain, gingival/serum oxidative markers and selected alveolar-bone measures favored H₂ water versus high-fat diet alone. Serum cholesterol/triglyceride comparisons between the two high-fat groups and trabecular number/thickness were not significant.
The complete free publisher article, tables and figures were checked for randomization, group size, measured H₂ decay, schedule, bone/oxidative outcomes, explicit null lipid and microarchitecture findings, funding and conflicts.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Only six male rats per group, high-fat model without induced periodontitis, unquantified water intake, no non-H₂ electrolyzed-water control and possible mediation by lower weight gain. Japanese public grants funded the work; authors declared no conflict and identified the commercial electrolyzer model.
Diet-related gingival oxidative stress and alveolar-bone changes in rats; it does not establish prevention of periodontal disease in people.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 28098768 · DOI: 10.3390/nu9010064
Free full article at the publisher.
Complete open-access publisher article and PubMed metadata; full-text extraction checked 9 August 2026.
10 August 2026
Help people check the facts before they buy.
Hydrogenology keeps 665 source-checked records accessible without advertising or product promotion.
Help us correct the recordReport a discrepancy
Send the exact difference between this page and the linked source. The study reference is attached automatically.