Hydrogen molecules (H2) improve perfusion recovery via antioxidant effects in experimental peripheral arterial disease
Fu J et al. · Mol Med Rep. 2018;18(6):5009–5015.
Study at a glance
Preclinical
Mice with experimental peripheral arterial disease and cultured endothelial cells
H₂-saturated drinking water; separate cells received H₂-saturated culture medium · The article describes saturated H₂ water; a numeric concentration has not yet been extracted.
Perfusion assessed through days 14 and 21; tissue assays included day 7
The article reports improved perfusion and vascular-density measures and less necrosis with H₂ water, alongside cell and biochemical findings.
Animal and cell evidence with surrogate vascular outcomes and no human participants. Exact H₂ concentration remains to be confirmed.
What kind of evidence is this?
Preclinical
Controlled mouse hindlimb-ischemia experiment plus endothelial-cell experiments
Cardiovascular and circulatory health
H₂-rich water
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Mice with experimental peripheral arterial disease and cultured endothelial cells
Principal figures report n=10 for perfusion and n=8 for histology; cell experiments used repeated assays
Perfusion assessed through days 14 and 21; tissue assays included day 7
H₂-saturated drinking water; separate cells received H₂-saturated culture medium
H₂ dissolved in water or culture medium — H₂ only, not Brown’s gas and not inhalation.
The article describes saturated H₂ water; a numeric concentration has not yet been extracted.
Dehydrogenized water in mice and matched cell-culture controls
Outcomes and reported result
Limb perfusion, necrosis, capillary and artery density, oxidative-stress markers, cGMP and endothelial tube formation
The article reports improved perfusion and vascular-density measures and less necrosis with H₂ water, alongside cell and biochemical findings.
Methods and principal results checked in the open-access full text; exact drinking-water concentration still requires extraction.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Animal and cell evidence with surrogate vascular outcomes and no human participants. Exact H₂ concentration remains to be confirmed.
Experimental hindlimb ischemia in mice, not people with peripheral arterial disease.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 30320393 · DOI: 10.3892/mmr.2018.9546
Open-access full text is available through PMC.
Open-access PMC full text and PubMed record.
10 August 2026
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