Protective Role of Hydrogen Gas on Oxidative Damage and Apoptosis in Intestinal Porcine Epithelial Cells (IPEC-J2) Induced by Deoxynivalenol: A Preliminary Study
Ji X, Zheng W, Yao W. · Toxins. 2019;12(1):E5.
Study at a glance
Preclinical
Intestinal porcine epithelial IPEC-J2 cells exposed to deoxynivalenol in vitro.
IPEC-J2 cells were cultured for 12 hours in freshly prepared H₂-saturated medium, with or without 5 μM deoxynivalenol. · Fresh H₂-saturated medium: 0.61 ± 0.03 mM; after 12 hours: 0.06 ± 0.00 mM. Source gas purity was ≥99.999% and is not the administered dissolved concentration.
12-hour cell treatment.
Article-reported result excerpt (24 words maximum): “However, H_2 efficiently reduced the LDH release ( p < 0.05) and increased cell viability ( p < 0.05) in the DON + H_2…” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The article declares no conflicts of interest.
What kind of evidence is this?
Preclinical
Controlled animal and cell study
Gastrointestinal health
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Intestinal porcine epithelial IPEC-J2 cells exposed to deoxynivalenol in vitro.
Cell-culture study; viability assays used six parallel wells per group, with assay-specific replicate counts elsewhere.
12-hour cell treatment.
IPEC-J2 cells were cultured for 12 hours in freshly prepared H₂-saturated medium, with or without 5 μM deoxynivalenol.
H₂ dissolved in cell-culture medium — in-vitro exposure, not inhalation or injection.
Fresh H₂-saturated medium: 0.61 ± 0.03 mM; after 12 hours: 0.06 ± 0.00 mM. Source gas purity was ≥99.999% and is not the administered dissolved concentration.
Nitrogen-saturated medium with vehicle, deoxynivalenol alone, H₂ medium alone and deoxynivalenol plus H₂ medium.
Outcomes and reported result
survival or mortality, clinical symptoms or functional scores, tumor growth or cancer markers, oxidative-stress and antioxidant markers, apoptosis or cell injury, metabolic laboratory measures
Article-reported result excerpt (24 words maximum): “However, H_2 efficiently reduced the LDH release ( p < 0.05) and increased cell viability ( p < 0.05) in the DON + H_2…” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
The complete machine-readable article was checked for source identity, methods, intervention quantities, results, tables, funding and conflict declarations. This public record is based on the full article.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The article declares no conflicts of interest.
Applies to the reported animal, cell or tissue model, not directly to patients.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 31861743 · DOI: 10.3390/toxins12010005
A free full article is available through PubMed Central.
Official PubMed/PMC full article; record re-audited and corrected 25 August 2026.
25 August 2026
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