Hydrogen-rich saline mitigates pressure-overload-induced cardiac hypertrophy and atrial fibrillation in rats via the JAK-STAT signalling pathway
Wang C, Pan Z. · J Int Med Res. 2020;48(8):300060520936415.
What kind of evidence is this?
Preclinical
Controlled rat abdominal-aortic-constriction experiment plus cultured cardiomyocyte experiments
Cardiovascular and circulatory health
Other forms
Not reported in this record.
Not reported in this record.
Methods at a glance
Rats with pressure overload and cultured neonatal rat cardiomyocytes with cardiotrophin-1-induced hypertrophy
81 rats: sham n=23, aortic-constriction n=19, low-dose H₂ saline n=19 and high-dose H₂ saline n=20
Six weeks in the rat experiment
0.6 mmol/L hydrogen-rich saline at 3 or 6 mL/kg intraperitoneally every day; cells received H₂-rich medium
H₂ dissolved in saline or culture medium — H₂ only, not Brown’s gas and not inhalation.
0.6 mmol/L dissolved H₂.
Not applicable — intraperitoneal saline and culture medium, not gas inhalation.
No O₂ was co-delivered as part of the intervention.
Sham, pressure-overload plus ordinary saline and cultured-cell controls
Outcomes and reported result
Heart and atrial weights, atrial-fibrillation inducibility and duration, fibrosis and JAK-STAT signaling
The article reports less cardiac hypertrophy, atrial fibrosis and atrial-fibrillation incidence and duration at both H₂-saline doses, with JAK-STAT down-regulation.
The complete open-access article was checked for concentration, allocation, doses, duration, principal results, funding and conflicts.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Preclinical pressure-overload model with two doses, induced atrial-fibrillation testing and mechanistic cell experiments. Authors declared no conflicts and no specific grant funding. It is not evidence for treating human hypertrophy or atrial fibrillation.
Pressure-overload cardiac remodeling in rats and cultured neonatal rat cardiomyocytes.
Not reported in this record.
Not reported in this record.
Not reported in this record.
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 32762484 · DOI: 10.1177/0300060520936415
Open-access full text is available through PMC.
Open-access PMC/publisher full text and PubMed record.
2026-08-10