Hydrogen-rich saline mitigates pressure-overload-induced cardiac hypertrophy and atrial fibrillation in rats via the JAK-STAT signalling pathway
Wang C, Pan Z. · J Int Med Res. 2020;48(8):300060520936415.
Study at a glance
Preclinical
Rats with pressure overload and cultured neonatal rat cardiomyocytes with cardiotrophin-1-induced hypertrophy
0.6 mmol/L hydrogen-rich saline at 3 or 6 mL/kg intraperitoneally every day; cells received H₂-rich medium · 0.6 mmol/L dissolved H₂.
Six weeks in the rat experiment
The article reports less cardiac hypertrophy, atrial fibrosis and atrial-fibrillation incidence and duration at both H₂-saline doses, with JAK-STAT down-regulation.
Preclinical pressure-overload model with two doses, induced atrial-fibrillation testing and mechanistic cell experiments. Authors declared no conflicts and no specific grant funding. It is not evidence for treating human hypertrophy or atrial fibrillation.
What kind of evidence is this?
Preclinical
Controlled rat abdominal-aortic-constriction experiment plus cultured cardiomyocyte experiments
Cardiovascular and circulatory health
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Rats with pressure overload and cultured neonatal rat cardiomyocytes with cardiotrophin-1-induced hypertrophy
81 rats: sham n=23, aortic-constriction n=19, low-dose H₂ saline n=19 and high-dose H₂ saline n=20
Six weeks in the rat experiment
0.6 mmol/L hydrogen-rich saline at 3 or 6 mL/kg intraperitoneally every day; cells received H₂-rich medium
H₂ dissolved in saline or culture medium — H₂ only, not Brown’s gas and not inhalation.
0.6 mmol/L dissolved H₂.
Sham, pressure-overload plus ordinary saline and cultured-cell controls
Outcomes and reported result
Heart and atrial weights, atrial-fibrillation inducibility and duration, fibrosis and JAK-STAT signaling
The article reports less cardiac hypertrophy, atrial fibrosis and atrial-fibrillation incidence and duration at both H₂-saline doses, with JAK-STAT down-regulation.
The complete open-access article was checked for concentration, allocation, doses, duration, principal results, funding and conflicts.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical pressure-overload model with two doses, induced atrial-fibrillation testing and mechanistic cell experiments. Authors declared no conflicts and no specific grant funding. It is not evidence for treating human hypertrophy or atrial fibrillation.
Pressure-overload cardiac remodeling in rats and cultured neonatal rat cardiomyocytes.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 32762484 · DOI: 10.1177/0300060520936415
Open-access full text is available through PMC.
Open-access PMC/publisher full text and PubMed record.
10 August 2026
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