Microbial hydrogen economy alleviates colitis by reprogramming colonocyte metabolism and reinforcing intestinal barrier
Ge L, Qi J, Shao B, Ruan Z, Ren Y, Sui S, Wu X, Sun X, Liu S, Li S, Xu C, Song W. · 2022.
Study at a glance
Preclinical
Mice with dextran-sulfate-sodium-induced colitis and associated colon-tissue analyses.
Intraperitoneal hydrogen-rich saline throughout the experimental period. · The checked article reports preparation and repeated dosing; no dose is inferred beyond its methods.
17-day protocol, with colitis induction during the final week.
The hydrogen-saline group was reported to have less experimental colitis and better barrier-related, microbiome and metabolic measures than untreated colitis controls.
Animal and mechanistic microbiome evidence only; numerous correlated endpoints and unclear blinded assessment.
What kind of evidence is this?
Preclinical
Controlled mouse and tissue study
Gastrointestinal health
Other forms
Positive preclinical signal.
Animal disease, tissue-barrier, microbiome and metabolic outcomes.
Methods
Mice with dextran-sulfate-sodium-induced colitis and associated colon-tissue analyses.
Mouse groups and assay-specific subsamples were checked; the article does not give one consolidated total across all experiments.
17-day protocol, with colitis induction during the final week.
Intraperitoneal hydrogen-rich saline throughout the experimental period.
H₂ dissolved in saline.
The checked article reports preparation and repeated dosing; no dose is inferred beyond its methods.
Control, vehicle, colitis and colitis-plus-hydrogen-saline groups.
Outcomes and reported result
Colitis severity, intestinal barrier structure, microbiota, short-chain fatty acids and colonocyte metabolism.
The hydrogen-saline group was reported to have less experimental colitis and better barrier-related, microbiome and metabolic measures than untreated colitis controls.
The full article was checked for methods, intervention details, outcomes, positive and null findings, funding and conflicts. This record does not imply medical efficacy.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Animal and mechanistic microbiome evidence only; numerous correlated endpoints and unclear blinded assessment.
The reported DSS mouse-colitis model only.
SYRCLE animal-study tool — preliminary
Some concerns
Controlled groups were used, while concealment, assessor blinding and multiplicity handling were not fully established.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 35025709 · DOI: 10.1080/19490976.2021.2013764
A free full article is available through PubMed Central.
Official PubMed Central full article and PubMed bibliographic record; source identity, methods, intervention, results, funding and conflicts checked 10 August 2026.
10 August 2026
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