Hydrogen-Rich Saline Attenuates Chronic Allodynia after Bone Fractures via Reducing Spinal CXCL1/CXCR2-Mediated Iron Accumulation in Mice
Wang Y et al. · Brain Sci. 2022;12(12):1610.
What kind of evidence is this?
Preclinical
Controlled mouse fracture experiment with dose-response and mechanistic comparisons
Musculoskeletal and pain research
Other forms
Not reported in this record.
Not reported in this record.
Methods at a glance
Mice with tibial fracture and intramedullary pinning
Behavioral comparisons commonly n=6/group and biochemical comparisons n=4/group; multiple mechanistic groups
Three daily injections on days 4–6, with follow-up of developing allodynia; separate established-pain test on day 14
Hydrogen-rich saline 1, 5 or 10 mL/kg intraperitoneally once daily on days 4–6; a separate experiment used one 10 mL/kg dose on day 14
H₂ dissolved in saline — H₂ only, not Brown’s gas and not inhalation.
Dose reported as saline volume per body weight; dissolved-H₂ concentration was not extracted as a numeric value.
Not applicable — injected saline, not gas inhalation.
No O₂ was co-delivered as part of the intervention.
Fracture mice receiving saline, sham controls and additional pathway-manipulation groups
Outcomes and reported result
Mechanical and cold allodynia, spinal CXCL1/CXCR2, transferrin receptor, iron and pathway experiments
Repeated 5 and 10 mL/kg doses, but not 1 mL/kg, reduced fracture-associated allodynia. A single 10 mL/kg day-14 dose produced a transient reduction; pathway experiments were also reported.
Dose-response, principal results and figures checked in the open-access full text; the many mechanistic groups have not been extracted outcome by outcome.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Preclinical fracture-pain model, small groups and multiple mechanistic experiments. The results do not establish analgesic benefit after human fractures.
Mouse fracture-associated allodynia, not people with fracture pain.
Not reported in this record.
Not reported in this record.
Not reported in this record.
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 36552070 · DOI: 10.3390/brainsci12121610
Open-access full text is available through PMC.
Open-access PMC full text and PubMed record.
2026-08-10