Hydrogen-Rich Saline Attenuates Chronic Allodynia after Bone Fractures via Reducing Spinal CXCL1/CXCR2-Mediated Iron Accumulation in Mice
Wang Y et al. · Brain Sci. 2022;12(12):1610.
Study at a glance
Preclinical
Mice with tibial fracture and intramedullary pinning
Hydrogen-rich saline 1, 5 or 10 mL/kg intraperitoneally once daily on days 4–6; a separate experiment used one 10 mL/kg dose on day 14 · Dose reported as saline volume per body weight; dissolved-H₂ concentration was not extracted as a numeric value.
Three daily injections on days 4–6, with follow-up of developing allodynia; separate established-pain test on day 14
Repeated 5 and 10 mL/kg doses, but not 1 mL/kg, reduced fracture-associated allodynia. A single 10 mL/kg day-14 dose produced a transient reduction; pathway experiments were also reported.
Preclinical fracture-pain model, small groups and multiple mechanistic experiments. The results do not establish analgesic benefit after human fractures.
What kind of evidence is this?
Preclinical
Controlled mouse fracture experiment with dose-response and mechanistic comparisons
Musculoskeletal and pain research
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Mice with tibial fracture and intramedullary pinning
Behavioral comparisons commonly n=6/group and biochemical comparisons n=4/group; multiple mechanistic groups
Three daily injections on days 4–6, with follow-up of developing allodynia; separate established-pain test on day 14
Hydrogen-rich saline 1, 5 or 10 mL/kg intraperitoneally once daily on days 4–6; a separate experiment used one 10 mL/kg dose on day 14
H₂ dissolved in saline — H₂ only, not Brown’s gas and not inhalation.
Dose reported as saline volume per body weight; dissolved-H₂ concentration was not extracted as a numeric value.
Fracture mice receiving saline, sham controls and additional pathway-manipulation groups
Outcomes and reported result
Mechanical and cold allodynia, spinal CXCL1/CXCR2, transferrin receptor, iron and pathway experiments
Repeated 5 and 10 mL/kg doses, but not 1 mL/kg, reduced fracture-associated allodynia. A single 10 mL/kg day-14 dose produced a transient reduction; pathway experiments were also reported.
Dose-response, principal results and figures checked in the open-access full text; the many mechanistic groups have not been extracted outcome by outcome.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical fracture-pain model, small groups and multiple mechanistic experiments. The results do not establish analgesic benefit after human fractures.
Mouse fracture-associated allodynia, not people with fracture pain.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 36552070 · DOI: 10.3390/brainsci12121610
Open-access full text is available through PMC.
Open-access PMC full text and PubMed record.
10 August 2026
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