Nrf2/HO-1-sulfiredoxin1 pathway involved in nanobubble hydrogen-dissolved water-mediated protective effects by ultrasound-guided early local injection in a rat model of chronic constriction injury-induced neuropathic pain
Yu J, Yin Z, Ma H, Zhang B, Meng C. · Frontiers in molecular neuroscience. 2025;18:1666575.
Study at a glance
Preclinical
Thirty-six rats in a chronic-constriction-injury model of neuropathic pain.
Ultrasound-guided local injection of low-concentration nanobubble H₂ water (1,300 ppb) or high-concentration nanobubble H₂ water (2,600 ppb), 5 mL/kg, either once on Day 1 or on Days 1, 3 and 5 after injury. · Low concentration: 1,300 ppb. High concentration: 2,600 ppb. Injection volume: 5 mL/kg.
One injection on Day 1 or three injections on Days 1, 3 and 5.
Article-reported result excerpt (24 words maximum): “After the nanobubble hydrogen-dissolved water was injected, the number of axons increased, and the distribution tended to be neat, which was most evident in…” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The full article contains a conflict, commercial-support or supplied-product declaration; consult the source wording.
What kind of evidence is this?
Preclinical
Controlled preclinical study
Musculoskeletal and pain research
H₂-rich water
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Thirty-six rats in a chronic-constriction-injury model of neuropathic pain.
36 rats randomized to six groups (n=6 each).
One injection on Day 1 or three injections on Days 1, 3 and 5.
Ultrasound-guided local injection of low-concentration nanobubble H₂ water (1,300 ppb) or high-concentration nanobubble H₂ water (2,600 ppb), 5 mL/kg, either once on Day 1 or on Days 1, 3 and 5 after injury.
H₂ dissolved in drinking water plus an injected or infused H₂ intervention — H₂ only unless the full text explicitly reports oxygen co-delivery.
Low concentration: 1,300 ppb. High concentration: 2,600 ppb. Injection volume: 5 mL/kg.
Sham and untreated chronic-constriction-injury groups, with one-dose and three-dose groups at each H₂ concentration.
Outcomes and reported result
clinical symptoms or functional scores, tumor growth or cancer markers, inflammation and cytokines, oxidative-stress and antioxidant markers, apoptosis or cell injury, metabolic laboratory measures
Article-reported result excerpt (24 words maximum): “After the nanobubble hydrogen-dissolved water was injected, the number of axons increased, and the distribution tended to be neat, which was most evident in…” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
The complete machine-readable article was checked for source identity, methods, intervention quantities, results, tables, funding and conflict declarations. This public record is based on the full article.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The full article contains a conflict, commercial-support or supplied-product declaration; consult the source wording.
Applies to the reported animal, cell or tissue model, not directly to patients.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 41479560 · DOI: 10.3389/fnmol.2025.1666575
A free full article is available through PubMed Central.
Official PubMed/PMC full article; record re-audited and corrected 25 August 2026.
25 August 2026
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