Nrf2/HO-1-sulfiredoxin1 pathway involved in nanobubble hydrogen-dissolved water-mediated protective effects by ultrasound-guided early local injection in a rat model of chronic constriction injury-induced neuropathic pain
Yu J, Yin Z, Ma H, Zhang B, Meng C. · Frontiers in molecular neuroscience. 2025;18:1666575.
What kind of evidence is this?
Preclinical
Controlled preclinical study
Musculoskeletal and pain research
H₂-rich water
Not reported in this record.
Not reported in this record.
Methods at a glance
rats, rat, tissue, tissues used to study musculoskeletal and pain research.
Preclinical study; the full text reports 36 rats, with group/assay counts including n=6, n=4.
Multiple article-specific time points; see the linked full methods.
Full text reports H₂ dissolved in drinking water plus an injected or infused H₂ intervention. Detected intervention quantities or schedules: 300 ppb, 600 ppb, 5 mL/kg.
H₂ dissolved in drinking water plus an injected or infused H₂ intervention — H₂ only unless the full text explicitly reports oxygen co-delivery.
H₂-related quantities reported in the intervention passages: 300 ppb, 600 ppb, 5 mL/kg. Values are not combined across different experimental arms.
Not applicable — no inhaled H₂ intervention was identified in the full article.
No inhaled O₂ co-delivery was identified for this non-inhalation intervention.
Control or comparison condition reported in the full article; see the linked methods for arm-specific details.
Outcomes and reported result
clinical symptoms or functional scores, tumor growth or cancer markers, inflammation and cytokines, oxidative-stress and antioxidant markers, apoptosis or cell injury, metabolic laboratory measures
Article-reported result excerpt (24 words maximum): “After the nanobubble hydrogen-dissolved water was injected, the number of axons increased, and the distribution tended to be neat, which was most evident in…” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
The complete machine-readable article was checked for source identity, methods, intervention quantities, results, tables, funding and conflict declarations. This public record is based on the full article.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The full article contains a conflict, commercial-support or supplied-product declaration; consult the source wording.
Applies to the reported animal, cell or tissue model, not directly to patients.
Not reported in this record.
Not reported in this record.
Not reported in this record.
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 41479560 · DOI: 10.3389/fnmol.2025.1666575
A free full article is available through PubMed Central.
Official Europe PMC JATS full article and PubMed/PMC identifiers; structured full-text extraction and validation completed 8 August 2026.
2026-08-10