Effects of Hydrogen-Rich Water on Interleukin-1β, Number of Osteoclasts and Osteoblasts in Streptozotocin-induced Diabetic Rats with Orthodontic Tooth Movement
Hadi LA, Hutapea AM, Florenly F. · Indonesian Biomedical Journal. 2023;15(6):376–383.
Study at a glance
Preclinical
Male rats with or without streptozotocin-induced diabetes and orthodontic tooth movement.
Hydrogen-rich water 5 mL by gavage three times daily for 4 weeks; corresponding groups received placebo water. · The article reports a hydrogen concentration of 1,000–1,100 ppm. This unusually high value is reproduced exactly and is not converted or corrected by Hydrogenology.
4 weeks of gavage after diabetes induction.
IL-1β was 140.07±5.14 pg/mL in diabetic-plus-orthodontic rats and 109.06±14.13 with added hydrogen-rich water. Osteoclast counts were lower and osteoblast counts higher in the combined group; the article reports p=0.000 for several comparisons.
Small preclinical study. The stated 1,000–1,100 ppm concentration is atypical for dissolved H₂ and is reported without correction; a unit or measurement issue cannot be excluded.
What kind of evidence is this?
Preclinical
Randomized controlled rat study
Oral and dental health
H₂-rich water
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Male rats with or without streptozotocin-induced diabetes and orthodontic tooth movement.
30 rats randomized to six groups; n=5 per group.
4 weeks of gavage after diabetes induction.
Hydrogen-rich water 5 mL by gavage three times daily for 4 weeks; corresponding groups received placebo water.
H₂ dissolved in water — H₂ only, not Brown's gas.
The article reports a hydrogen concentration of 1,000–1,100 ppm. This unusually high value is reproduced exactly and is not converted or corrected by Hydrogenology.
Orthodontic, hydrogen-water, diabetic, diabetic-plus-orthodontic, diabetic-plus-hydrogen-water and combined groups.
Outcomes and reported result
Serum IL-1β and osteoclast/osteoblast counts at orthodontic pressure and tension sides.
IL-1β was 140.07±5.14 pg/mL in diabetic-plus-orthodontic rats and 109.06±14.13 with added hydrogen-rich water. Osteoclast counts were lower and osteoblast counts higher in the combined group; the article reports p=0.000 for several comparisons.
The free journal full article, methods, tables and figures were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Small preclinical study. The stated 1,000–1,100 ppm concentration is atypical for dissolved H₂ and is reported without correction; a unit or measurement issue cannot be excluded.
Diabetic rat orthodontic-tooth-movement model only.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
OpenAlex ID: W4389568527 · DOI: 10.18585/inabj.v15i6.2677
Open-access journal full article.
Indonesian Biomedical Journal full article; full-text extraction checked 8 August 2026.
10 August 2026
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