Molecular hydrogen suppresses activated Wnt/β-catenin signaling
Lin Y, Ohkawara B, Ito M, Misawa N, Miyamoto K, Takegami Y, Masuda A, Toyokuni S, Ohno K. · Scientific reports. 2016;6:31986.
Study at a glance
Preclinical
cell, cells, mouse, rabbit used to study oral and dental health.
Full text reports H₂ dissolved in drinking water. Detected intervention quantities or schedules: 1 ml, 100 ml, 100%, 5%, 10%, 50%, 30 mM, 4 μM, 2 μM, 2 min. · H₂-related quantities reported in the intervention passages: 1 ml, 100 ml, 100%, 5%, 10%, 50%, 30 mM, 4 μM, 2 μM, 2 min. Values are not combined across different experimental arms.
2 min
Article-reported result excerpt (24 words maximum): “Addition of Wnt3a or a GSK3 inhibitor, LiCl or BIO, to the culture medium for 24 h increased Topflash reporter activity, which, however, was…” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. No explicit conflict statement was identified in the machine-readable full article; absence is not inferred.
What kind of evidence is this?
Preclinical
Randomized blinded controlled study
Oral and dental health
H₂-rich water
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
cell, cells, mouse, rabbit used to study oral and dental health.
Preclinical study; group or assay counts reported in the full text include n=3, n=4, n=6, n=9, n=5.
2 min
Full text reports H₂ dissolved in drinking water. Detected intervention quantities or schedules: 1 ml, 100 ml, 100%, 5%, 10%, 50%, 30 mM, 4 μM, 2 μM, 2 min.
H₂ dissolved in drinking water — H₂ only unless the full text explicitly reports oxygen co-delivery.
H₂-related quantities reported in the intervention passages: 1 ml, 100 ml, 100%, 5%, 10%, 50%, 30 mM, 4 μM, 2 μM, 2 min. Values are not combined across different experimental arms.
Control or comparison condition reported in the full article; see the linked methods for arm-specific details.
Outcomes and reported result
clinical symptoms or functional scores, tumor growth or cancer markers, oxidative-stress and antioxidant markers, apoptosis or cell injury, metabolic laboratory measures, neurological or behavioral measures
Article-reported result excerpt (24 words maximum): “Addition of Wnt3a or a GSK3 inhibitor, LiCl or BIO, to the culture medium for 24 h increased Topflash reporter activity, which, however, was…” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
The complete machine-readable article was checked for source identity, methods, intervention quantities, results, tables, funding and conflict declarations. This public record is based on the full article.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. No explicit conflict statement was identified in the machine-readable full article; absence is not inferred.
Applies to the reported animal, cell or tissue model, not directly to patients.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 27558955 · DOI: 10.1038/srep31986
A free full article is available through PubMed Central.
Official PubMed/PMC full article; source identity, intervention data and declarations re-audited 25 August 2026.
25 August 2026
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