The effect of hydrogen gas evolution of magnesium implant on the postimplantation mortality of rats
Noviana D, Paramitha D, Ulum MF, Hermawan H. · Journal of Orthopaedic Translation. 2016;5:9–15.
Study at a glance
Preclinical
Twenty adult 12-week-old Sprague–Dawley rats receiving porous magnesium implantation or sham surgery.
A 0.24–0.26 g porous pure-magnesium tablet was implanted into a femoral bone defect and left to degrade without puncturing the gas cavity. · Implant mass was about 1.7% of rat body weight and explicitly considered high; H₂ production rate, concentration and total volume were not quantified.
Eighteen days after implantation, with radiography on days 7 and 14 and blood measures on day 7.
A large gas cavity formed rapidly, spread from muscle into subcutaneous compartments and all magnesium-implant rats had died by day 18, whereas sham animals survived. Several blood indices changed, while RBC/WBC and other listed measures were not significant. Bone-defect radiodensity change in sham animals was not significant. The design shows harm from this high-burden implant condition, not harm from ordinary therapeutic H₂ exposure.
Small unequal groups, exceptionally large rapidly corroding implant relative to rat mass, gas not chemically sampled/quantified, no gas evacuation and cause of death inferred rather than directly established. Indonesian/Canadian public/institutional support was reported; authors declared no conflicts.
What kind of evidence is this?
Preclinical
Controlled rat femoral-implant safety experiment
Musculoskeletal and pain research
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Twenty adult 12-week-old Sprague–Dawley rats receiving porous magnesium implantation or sham surgery.
Magnesium implant n=15; sham surgery n=5.
Eighteen days after implantation, with radiography on days 7 and 14 and blood measures on day 7.
A 0.24–0.26 g porous pure-magnesium tablet was implanted into a femoral bone defect and left to degrade without puncturing the gas cavity.
H₂ generated locally by magnesium corrosion in tissue — not Brown's gas and not inhaled/dissolved H₂ therapy.
Implant mass was about 1.7% of rat body weight and explicitly considered high; H₂ production rate, concentration and total volume were not quantified.
Identical femoral surgery without magnesium implantation.
Outcomes and reported result
Survival, visible/radiographic gas-cavity spread, defect radiodensity and peripheral blood-cell indices.
A large gas cavity formed rapidly, spread from muscle into subcutaneous compartments and all magnesium-implant rats had died by day 18, whereas sham animals survived. Several blood indices changed, while RBC/WBC and other listed measures were not significant. Bone-defect radiodensity change in sham animals was not significant. The design shows harm from this high-burden implant condition, not harm from ordinary therapeutic H₂ exposure.
The complete free PMC article, methods, figures/table and disclosures were checked for implant burden, group sizes, missing H₂ quantification, mortality/gas distribution, positive and null findings, funding and conflicts.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Small unequal groups, exceptionally large rapidly corroding implant relative to rat mass, gas not chemically sampled/quantified, no gas evacuation and cause of death inferred rather than directly established. Indonesian/Canadian public/institutional support was reported; authors declared no conflicts.
Implant-material safety warning in rats; it does not evaluate inhaled H₂ or ordinary H₂-water exposure.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 30035070 · DOI: 10.1016/j.jot.2015.08.003
Free full article in PubMed Central.
Complete PMC article and PubMed metadata; full-text extraction checked 9 August 2026.
10 August 2026
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