Hydrogen saline water accelerates fracture healing by suppressing autophagy in ovariectomized rats
Guo J, Tian S, Wang Z, Wang Y, Zhang X, Zhang Y, Hou Z, Dong W. · 2022.
Study at a glance
Preclinical
Ovariectomized rats with experimental fracture and related cell assays.
Hydrogen-rich saline/water during fracture healing. · The article reports approximately 1.6 ppm for the hydrogen preparation.
Article-specific fracture-healing follow-up.
The hydrogen-treated condition was reported to have faster fracture-healing measures and altered autophagy-related signaling.
Animal and cell evidence only; multiple mechanistic outcomes and incomplete blinding information.
What kind of evidence is this?
Preclinical
Controlled rat and cell study
Musculoskeletal and pain research
Other forms
Positive preclinical signal.
Bone-healing, tissue and mechanistic cell outcomes.
Methods
Ovariectomized rats with experimental fracture and related cell assays.
24 rats plus cell experiments.
Article-specific fracture-healing follow-up.
Hydrogen-rich saline/water during fracture healing.
H₂ dissolved in saline or water as described by the article.
The article reports approximately 1.6 ppm for the hydrogen preparation.
Fracture-model controls and hydrogen-treatment conditions.
Outcomes and reported result
Callus formation, bone measures, histology, autophagy and cell assays.
The hydrogen-treated condition was reported to have faster fracture-healing measures and altered autophagy-related signaling.
The full article was checked for methods, intervention details, outcomes, positive and null findings, funding and conflicts. This record does not imply medical efficacy.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Animal and cell evidence only; multiple mechanistic outcomes and incomplete blinding information.
The reported ovariectomized-rat fracture model only.
SYRCLE animal-study tool — preliminary
Some concerns
Controls were reported, but allocation concealment and blinded outcome assessment were unclear.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 36120426 · DOI: 10.3389/fendo.2022.962303
A free full article is available through PubMed Central.
Official PubMed Central full article and PubMed bibliographic record; source identity, methods, intervention, results, funding and conflicts checked 10 August 2026.
10 August 2026
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