A more accurate indicator to evaluate oxidative stress in rat plasma with osteoporosis[†]
Dong WC, Guo JL, Jiang XH, Xu L, Wang H, Ni XY, Zhang YZ, Zhang ZQ, Jiang Y. · 2023.
Study at a glance
Preclinical
Rats in an ovariectomy-associated osteoporosis model.
Hydrogen-rich saline during the model protocol. · About 1.6 ppm; article-specific volume and schedule.
Four-week intervention.
Selected plasma redox ratios were reported to differ between ovariectomy controls and the hydrogen-saline group.
Surrogate biomarker study with no fracture or functional endpoint and small analytical subsets.
What kind of evidence is this?
Preclinical
Controlled rat biomarker study
Musculoskeletal and pain research
Other forms
Exploratory positive preclinical biomarker signal.
Animal laboratory biomarker outcomes.
Methods
Rats in an ovariectomy-associated osteoporosis model.
Assay-specific groups of approximately 12 rats with smaller analytical subsets.
Four-week intervention.
Hydrogen-rich saline during the model protocol.
H₂ dissolved in saline.
About 1.6 ppm; article-specific volume and schedule.
Normal, ovariectomy and hydrogen-treatment groups.
Outcomes and reported result
Plasma redox-pair and oxidative-stress measurements.
Selected plasma redox ratios were reported to differ between ovariectomy controls and the hydrogen-saline group.
The full article was checked for methods, intervention details, outcomes, positive and null findings, funding and conflicts. This record does not imply medical efficacy.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Surrogate biomarker study with no fracture or functional endpoint and small analytical subsets.
The reported rat osteoporosis model only.
SYRCLE animal-study tool — preliminary
Some concerns
Comparator groups were used, but allocation, assay blinding and selective-marker reporting were unclear.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 36686958 · DOI: 10.1039/d2ra05572d
A free full article is available through PubMed Central.
Official PubMed Central full article and PubMed bibliographic record; source identity, methods, intervention, results, funding and conflicts checked 10 August 2026.
10 August 2026
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